川井 夫規子, 伊東 紘一, 谷口 信行, 中村 みちる

Fukiko KAWAI, Kouichi ITOH, Nobuyuki TANIGUCHI, Michiru NAKAMURA

自治医科大学臨床病理学

Department of Clinical Pathology, Jichi Medical School

キーワード : Echo intensity histogram, Frequency dependent attenuation (FDA), Rat, Siderotic liver, Ultrasonic tissue characterization

We used iron-overloaded rats to see if iron content in siderotic livers could be estimated from hepatic frequency dependent attenuation (FDA) or echo level. Siderotic livers were produced either by feeding rats a diet containing 0.8% 8-hydoxyquinoline and 2.19% iron (II) fumarate of by subcutaneous injection of iron dextran twice weekly. FDA was measured by the spectral-shift zero-crossing method using a 7.5 MHz transducer. Echo levels were determined from echo intensity histograms by subtracting echo intensity of a standard graphite-agar phantom from that of a sample liver. Degree of iron deposition in the liver was graded microscopically, and iron content was quantitated according to the method of Foy. To exclude the influence of lipid or water content, lipid was assayed according to the method of Folch; water, by the freeze-dry method. No difference was noted in content of either substance when compared with that of normal controls. FDA correlated closely with iron content (r=0.882, p