山下 裕一^{1, 2}, 黒肱 敏彦^{1, 2, 3}, 甲斐 良樹¹, 渡辺 建詞¹, 立花 克郎^{1, 2, 3}, 白日 高歩^{1, 2}

Yuichi YAMASHITA^{1, 2}, Toshihiko KUROHIJI^{1, 2, 3}, Yoshiki KAI¹, Kenshi WATANABE¹, Katsuro TACHIBANA^{1, 2, 3}, Takayuki SHIRAKUSA^{1, 2}

¹福岡大学第2外科, ²天神会古賀病院, ³福岡大学第1内科

¹Second Department of Surgery, Fukuoka University, School of Medicine, ²Koga Hospital, ³First Department of Internal Medicine, Fukuoka University, School of Medicine

キーワード : Cell line, Gastric cancer, Porfimer sodium (Photofrin), Sonodynamic therapy , Ultrasound

We determined the conditions of temperature and light intensity for sonodynamic chemotherapy on a human gastric cancer cell line (KATO-Ⅲ). The drug used in this study was porfimer sodium (Photofrin; Pf), and the cells were exposed to 0.9 MHz ultrasound. The ultrasound transducer used was driven at an intensity of 0.5 W/cm² for 10 seconds. Cells were held 10℃ or 25℃ to evaluate the effect of temperature, and a dimly lighted room and a dark room were used for evaluating light intensity. Cell-killing rate under these various conditions by treatment with ultrasound, Pf, or both, were evaluated statistically. A combination of ultrasound and Pf killed cells at a higher rate under all four sets of conditions than ultrasound alone. After administration of 50 μg/ml of Pf, cell-killing rate in a dimly lighted or dark room was significantly higher at 25℃ than at 10℃ (p